PMID- 2988516
OWN - NLM
STAT- MEDLINE
DA  - 19850724
DCOM- 19850724
LR  - 20091118
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 228
IP  - 1
DP  - 1985 May 15
TI  - The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal
      sequence and interaction with 6 beta-halogenopenicillanates.
PG  - 241-8
AB  - The beta-lactamase of Enterobacter cloacae P99 consists of one polypeptide chain 
      of Mr 39000 devoid of disulphide bridges and free thiol groups. It contains an
      unusually high proportion of tyrosine and tryptophan. The N-terminal sequence
      exhibits overlaps with the tryptic peptide obtained after labelling the active
      site with 6 beta-iodopenicillanate. The active-site serine residue is at position
      64. The homology with the chromosomal beta-lactamase of Escherichia coli K 12
      (ampC gene) is lower within the 25 residues of the N-terminal portion than around
      the active-site serine residue. The P99 beta-lactamase is inactivated by 6
      beta-bromo- and 6 beta-iodo-penicillanate, with a second-order rate constant of
      110-140M-1 X s-1 at 30 degrees C and pH 7.0, a value that is much lower than that
      observed with class-A beta-lactamases.
FAU - Joris, B
AU  - Joris B
FAU - De Meester, F
AU  - De Meester F
FAU - Galleni, M
AU  - Galleni M
FAU - Reckinger, G
AU  - Reckinger G
FAU - Coyette, J
AU  - Coyette J
FAU - Frere, J M
AU  - Frere JM
FAU - Van Beeumen, J
AU  - Van Beeumen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - ENGLAND
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Amino Acids)
RN  - 26631-90-3 (6-bromopenicillanic acid)
RN  - 73-22-3 (Tryptophan)
RN  - 74772-32-0 (6-iodopenicillanic acid)
RN  - 87-53-6 (Penicillanic Acid)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Enterobacter/*enzymology
MH  - Enterobacteriaceae/*enzymology
MH  - Kinetics
MH  - Penicillanic Acid/*metabolism
MH  - Spectrophotometry
MH  - Tryptophan/analysis
MH  - *beta-Lactamases/antagonists & inhibitors
PMC - PMC1144975
OID - NLM: PMC1144975
EDAT- 1985/05/15
MHDA- 1985/05/15 00:01
CRDT- 1985/05/15 00:00
PST - ppublish
SO  - Biochem J. 1985 May 15;228(1):241-8.